The SVF contains HSPCs that can home to the BM of irradiated recipients. (A) Experimental scheme. After lethal irradiation, the mice underwent transplantation with control buffer (control), 5 × 10⁶ GFP⁺ SVF (SVFT) cells, or 5 × 10⁶ GFP⁺ BM (BMT) cells. At 24 hours after transplantation, femurs were sampled. (B) The femurs were cryosectioned, immunostained for PECAM-1 (for vasculature) and stained with Hoechst dye (for nuclei), and viewed by fluorescent microscopy. Scale bars, 100 μm. (C) Representative flow cytometric profiles of BM cells from control, SVFT, and BMT recipients. Numbers indicate the occurrence of GFP⁺ cells among 10⁶ live BM cells. (D) Percentage of GFP⁺ cells in the BM is presented. Bars represent mean ± SD of 4 mice per group. *P < .05 vs BM. (E) The BM cells of SVFT recipients were plated on methylcellulose-based medium, and cultured cells gave rise to GFP⁺ colonies.