HSPCs in the SVF are capable of long-term reconstitution in hematopoiesis in recipient mice. (A) Experimental scheme. After sublethal irradiation, the mice underwent transplantation with control buffer (control), 5 × 10⁶ of GFP⁺ SVF (SVFT) cells, or 5 × 10⁶ GFP⁺ BM (BMT) cells. At 16 weeks after transplantation, blood and several organs were sampled. (B) The percentage of donor-derived GFP⁺ cells in PBMCs was analyzed by flow cytometry. Bars represent the mean (n = 7 per group), and each ■ (SVFT) and ▲ (BMT) represents 1 sample. (C) Representative flow cytometric profiles of PBMCs in primary (SVFT) and secondary recipients (BMT from the primary SVFT). (D) Changes of percentages of GFP⁺ cells in PBMCs from the primary recipients to the secondary recipients were plotted (dotted lines). ■ represents the multilineage-reconstitution recipients and ● represents T lymphocyte–dominant reconstitution recipients. Blue bars represent the mean (n = 7 per group). *P < .05 vs primary recipients. (E) Immunostaining for CD3 (T lymphocyte), B220 (B lymphocyte), Gr-1 (myeloid cell), and Mac-1 (myeloid cell) and the counter-staining of nuclei with Hoechst dye in the indicated organs. White arrows indicate blood cells derived from SVF. Scale bars, 20 μm.