HSPCs in the SVF increased upon promotion of HSPC mobilization from the BM. (A-B) Comparisons of the percentage of LSK cells and CD150⁺CD48⁻LSK cells in the SVF treated with control buffer (control) or cyclophosphamide/G-CSF (G-CSF). Bars indicate mean ± SD (n = 4 per each group). *P < .05 vs control. (C-D) CFC assay of 5 × 10⁵ SVF cells treated with control or G-CSF. The number of total colonies and each type of colony was counted. Bars represent mean ± SD (n = 4). *P < .05 vs control. (E) Percentage of donor-derived GFP⁺ cells in PBMCs was analyzed by flow cytometry. Bars represent mean, and each ■ (control, n = 5) and ▲ (G-CSF, n = 6) represents 1 sample. (F) Representative flow cytometric profiles show long-term multilineage reconstitution in the blood of primary recipients that underwent transplantation with G-CSF–treated SVF.