Figure 7
The in vivo sensitivity of 6812/2 to SMI-4a treatment. (A) To examine the effect of treatment on tumor growth, 18 Nu/nu mice were subcutaneously injected with 2 × 106 cells of 6812/2 on day 0. Mice were then randomly assigned to 3 groups, vehicle, SMI-4a once daily, or SMI-4a twice daily and treatment was initiated on day 3. Tumor size was measured by calipers on day 0, 3, 7, 10, 14, 17, and 21, and calculated as described in “Mouse models.” The statistical difference in the tumor sizes was determined using Mann-Whitney U test. (B) The levels of p70 S6K phosphorylation, total p70 S6K, phospho ERK, total ERK, MYC, and GAPDH were determined by Western blotting in control (V2, V4, and V5) or treated tumors (D1, D2, and D7).
