Figure 6
Figure 6. Combined pretreatment with KGF + PFT-β additively restores numbers of total and naive CD4+ and CD8+ T cells in LN by 6 weeks after allogeneic BMT. Lethally irradiated CB6F1 recipients of allogeneic (BALB/c) BM were left untreated (BMT Control) or pretreated with KGF, PFT-β, or KGF + PFT-β and analyzed for the presence of T cells in the LNs at 6 weeks after BMT alongside unmanipulated age-/sex-matched CB6F1 controls (non-BMT Control). Mean absolute numbers ± SEMs of (A) total CD4+CD3+ T cells, (B) naive (CD62LhighCD44low) CD4+ T cells, (C) total CD8+CD3+ T cells, (D) naive (CD62LhighCD44low) CD8+ T cells, and (E) CD4+CD3−CD11c−B220− Lti cells in the LNs are shown. LN cells were pooled from inguinal, axillary, and mesenteric LNs. Data are representative of 2 experiments, each with 4 mice per group; *P < .05.

Combined pretreatment with KGF + PFT-β additively restores numbers of total and naive CD4+ and CD8+ T cells in LN by 6 weeks after allogeneic BMT. Lethally irradiated CB6F1 recipients of allogeneic (BALB/c) BM were left untreated (BMT Control) or pretreated with KGF, PFT-β, or KGF + PFT-β and analyzed for the presence of T cells in the LNs at 6 weeks after BMT alongside unmanipulated age-/sex-matched CB6F1 controls (non-BMT Control). Mean absolute numbers ± SEMs of (A) total CD4+CD3+ T cells, (B) naive (CD62LhighCD44low) CD4+ T cells, (C) total CD8+CD3+ T cells, (D) naive (CD62LhighCD44low) CD8+ T cells, and (E) CD4+CD3CD11cB220 Lti cells in the LNs are shown. LN cells were pooled from inguinal, axillary, and mesenteric LNs. Data are representative of 2 experiments, each with 4 mice per group; *P < .05.

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