Pretreatment with KGF and PFT-β significantly improves immune clearance of Lm after allogeneic BMT. Lethally irradiated CB6F1 recipients of allogeneic (balb/c) T cell–depleted BM were left untreated (BMT Control) or pretreated with KGF, PFT-β, or KGF + PFT-β and immunized at 4 weeks after BMT alongside unmanipulated age-/sex-matched CB6F1 controls (non-BMT Control). For primary immunization, 5 × 104 CFU of Lm (strain 2C) was injected intravenously. Immunized mice were rechallenged with 2 × 106 CFU of Lm-2C, 5 weeks after primary infection. Then after 3 days, bacterial CFUs in liver (A) and spleen (B) were determined by plating of serial dilutions of organ homogenates onto BHI agar. Data are representative of 2 experiments, each with 4 mice per group. † indicates mouse succumbed to infection; *P < .05 compared with BMT controls.
