Phagocyte recruitment in the peritoneal cavity is inhibited in Hck^−/− mice. (A) Total inflammatory cell recruitment in wt and Hck^−/− mice after thioglycollate injection in the peritoneal cavity (n = 4-10 mice per group). (B) Recruitment of macrophages and neutrophils is decreased in Hck^−/− mice 24 and 72 hours after thioglycollate injection (n = 3). At 0 hours, neutrophils are absent in the peritoneal cavity and resident macrophages are more numerous in wt mice. (C) Immunohistochemistry of macrophages (F4/80 antibodies) performed before and 72 hours after thioglycollate injection. Micrographs show a representative experiment of 3; macrophages are accumulating in peritoneal tissue of Hck^−/− mice and no apoptotic cell is noticed. Original magnification ×100 (insets: ×400). Asterisks indicate areas of macrophage accumulation. (D) Quantification of macrophage and neutrophil distribution in peritoneal tissue from wt and Hck^−/− mice (expressed as the number of immunostained cells/mm² of peritoneal tissue). *P < .05; **P < .01; ***P < .001.