Protease-dependent macrophage migration through Matrigel. (A) Scanning electron microscopy images showing wt and Hck^−/− BMDMs (green) on thick Matrigel (brown) transwells in the presence or absence of protease inhibitors. Matrigel remodeling (arrows) is more important with wt than Hck^−/− BMDMs and is inhibited by protease inhibitors. Images are showing BMDMs infiltrating the matrix and are representative of 3 experiments (scale bar represents 10 μm). (B) Protease inhibitors inhibit both wt and Hck^−/− BMDMs migration through Matrigel. Results are expressed as the percentage of cells that migrate through Matrigel (mean ± SEM; n = 4). ***P < .001.