Effect of RTX on BCR-signaling components. (A) DOHH2 cells were preincubated with F(ab′)2 RTX (10 μg/mL, 16 hours) and stimulated with anti-IgG antibody (10 μg/mL, 5 or 15 minutes). Results are representative of 3 independent experiments. (B) Calcium mobilization was analyzed by flow cytometry. RL cells were preincubated with RTX (10 μg/mL, 16 hours), and calcium flux was stimulated by the addition of anti-IgM antibody (10 μg/mL). Ionomycin (2μM) was used as a positive control. Results are represented as ratio of indo-1 bound/indo-1 free and are representative of 3 independent experiments.