Short-term induction of AKT signaling causes expansion of the LSK compartment and increased LSK cycling. BMT assay with myr-AKT-ER or vector control retrovirus, followed by tamoxifen induction after engraftment for 3 days. (A) Multiparameter flow cytometric analysis of the Thy1.1-gated LSK and progenitor BM populations. The experiment was repeated 3 times, with 3 or 4 mice per group in each experiment. (B) Analysis of the Thy1.1-gated CD34⁻LSK BM subpopulation of myr-AKT-ER mice and vector control mice. The Student paired 2-tailed t test was used to compare the percentage Thy1.1⁺ CD34⁻ LSK cells between samples. (C) Cell-cycle analysis of Thy1.1-gated BM LSKs from myr-AKT-ER mice or vector control mice. Hoechst and pyronin Y were used to resolve the G₀, G₁, and S/G₂/M stages of the cell cycle. The experiment was repeated 3 times, with 2 to 4 mice per group in each experiment.