Expression of BST-2 in human non-Hodgkin lymphoma. BST-2 was detected in vascular structures of aggressive (A-C) and indolent (D-E) human non-Hodgkin lymphoma specimens using alkaline phosphatase–antialkaline phosphatase immunohistochemistry. Representative images of diffuse large B-cell lymphoma (A), Burkitt lymphoma (B), mantle cell lymphoma (C), follicular lymphoma (D), and chronic lymphocytic leukemia (E) are presented. Negative controls omitting the primary antibody were consistently negative (F). Although in most cases a predominant vascular staining was observed, also tumor cells were strongly immunostained in a case of Burkitt lymphoma (B). Slides were viewed with an Axiovert S100TV microscope (Carl Zeiss) using a 20×/0.40 Korr Ph2 objective and Glycergel Mounting Medium (Dako). Images were acquired using an AxioCam color camera and AxioVision software Version 4.7.1.0 (Carl Zeiss). Images were manipulated using ImageJ, Version 1.42q (available at http://rsb.info.nih.gov/ij/; A-F). Confocal images illustrate the colocalization of BST-2 and von Willebrand factor (vWF), exemplified on a section of chronic lymphocytic leukemia (G-I). Scale bars represent 100 μm (A-F) and 50 μm (G-I). Slides were viewed with an LSM510 Meta confocal microscope (Carl Zeiss) using 10×/0.45 W and 40×/1.2 W water objectives and Fluorescent Mounting Medium (Dako). Images were acquired with the LSM510 Meta confocal laser scanning microscope and software provided by the manufacturer (Carl Zeiss). Images were manipulated using ImageJ software, Version 1.42q (available at http://rsb.info.nih.gov/ij/; G-I).