Cbfb-MYH11 causes Cbfb/Runx1 repression–independent defects during primitive hematopoiesis. (A) Representative FACS plots of Ter119 and c-Kit staining of the primitive blood from embryos of the indicated ages and genotypes. Percentage of cells in each gate is given. Line graphs of the percentage (%) of (B) BrdU-positive (+) and (C) annexin V⁺, 7AAD-low cells in the peripheral blood of littermate embryos of the indicated genotypes and ages. Bar graphs of the percentage of (D) BrdU⁺ and (E) annexin V⁺, 7AAD-low cells in the peripheral blood of Cbfb^+/+ and Cbfb^−/− littermate embryos of the indicated ages. Data from age-matched Cbfb^+/MYH1 embryos was included for comparison. *Statistically significant difference (P < .05) compared with Cbfb^+/+ embryos; **statistically significant difference (P < .05) compared with Cbfb^+/+ and Cbfb^−/− embryos. N ≥ 3 for all genotypes and ages. Error bars indicate SD within each genotype.