IE adhesion to endothelium is dependent on binding of platelets to VWF strings, which can be cleaved by recombinant ADAMTS-13. (A) Rag-35 blocks platelets binding to VWF strings in a dose-dependent manner (n = 3). The control antibody (Rag-50) does not inhibit binding (n = 2). (B) Strings can be rapidly cleaved from the endothelium by ADAMTS-13 (added at t = 5 minutes) in the wash media (n = 3). All strings were removed after 15 minutes. ND indicates no detectable strings. *P < .05. ***P < .001.