Alox15 mice exhibit multiple hematopoietic defects. (A) Reduction in WBC, lymphocytes, and monocytes and an increase in basophils in 12- to 15-week-old wild-type Alox15 compared with B6 mice as an average (± SEM) of n = 4. (B-C) Defective B-cell development in Alox15 mice. (B) Splenocytes were isolated and stained for B cells (B220⁺) and T cells (CD3⁺). The percentage of B cells and T cells is indicated as the percentage of total cells for a representative experiment of n = 5. (C) BM cells were stained for B-cell progenitors and analyzed by flow cytometry: LSK, CLP (Lin⁻IL-7Rα⁺cKit^loSca1^lo), pre-pro-B (B220⁺CD43⁺HSA⁻CD19⁻), pro-B (B220⁺CD43⁺HSA⁺CD19⁺), pre-B (B220⁺CD43⁻IgM⁻CD19⁺), and immature B (B220⁺CD43⁻IgM⁺CD19⁺) cells as an average (± SEM) of n = 3. (D) Defect in Alox15 T-cell development. Thymocytes were stained for T-cell progenitors and analyzed by flow cytometry: ETP (Lin⁻CD25⁻cKit⁺), DN2 (Lin⁻CD25⁺cKit⁺), DN3 (Lin⁻CD25⁺cKit⁻), and double positive (CD4⁺CD8⁺) as an average (± SEM) of n = 3. (E) Hematologic analysis of peripheral blood from 12- to15-week-old B6 and Alox15 mice. The number of RBC, reticulocytes, hemoglobin (HGB), and the mean cell volume of the RBCs (MCV-RBC) in B6 and Alox15 is depicted as the average (± SEM) of n = 4. (F) Stress erythropoiesis in Alox15 mice demonstrated by expansion of erythroid progenitors. Proerythrocytes (CD71⁺Ter119^lo) and erythrocytes (Ter119^hi) in BM and spleen are indicated as a percentage of total cells for a representative experiment and a summary of 4 experiments; *P < .05, **P < .01 compared with wild-type.