Platelet aggregation and hemostasis are defective in platelets from Unc13d^Jinx mice. (A-G) Platelets from wild-type (WT, black) and Unc13d^Jinx mice (Unc13d^Jinx, gray) were prepared as described and diluted to approximately 3 × 10⁸/mL. CaCl₂ was added to each sample (0.7mM final) and platelets were activated with the indicated agonists. Increase in light transmittance (aggregation) and luminescence (ATP release) were monitored and plotted as a function of time. (H) The tails of wild-type (■) and Unc13d^Jinx (▴) mice were clipped and the duration of bleeding was measured (Bleeding Time) in seconds. The individual bleeding times (points) and averages (line) from n = 20 mice were plotted. A Student t test (SigmaPlot 9.0) was used to assess the statistical significance of the differences between groups.