Cytolysis in T2 peptide titration and specific tumor recognition of Hu CD8⁺ CTLs transduced with a p53(264-272)A2.1-specific scTCR. (A) Cytotoxicity of p53-specific Mu WT TCR p53 (●), Mu scTCR p53 (□), Mu scTCR p53/Mu Cα (▴), or Mock (○) transduced Hu CD8⁺ T cells in response to peptide-pulsed T2 targets at the indicated effector to target ratios (CD8⁺Vβ3⁺:T). (B) p53(264-272)A2.1-specific, TCR-transduced Hu CD8⁺ CTLs were tested at the indicated CD8⁺Vβ3⁺ to target ratios (CD8⁺Vβ3⁺:T) for cytolysis in response to p53⁺A2.1⁺ (Saos-2/143, UoC B11, BV 173, MZ 1851, and JY), p53⁻A2.1⁺ (Saos-2), p53⁺A2.1⁻ (Uoc B1), and p53⁻A2.1⁻ (K562) human tumor targets. The percentages of lysis are calculated as means from replicates and are shown for a representative chromium release assay of 2 experiments.