LMO2 protein expression in t(17;19)-ALL. (A) Western blot analysis of LMO2. Relative expression of each cell line was determined by quantifying the intensity of each band using KOPT6, a T-ALL cell line with t(11;14), as a positive control and KOPT-5, a T-ALL cell line without LMO2 expression, as a negative control, and normalized by the level of α-tubulin expression as an internal control. (B) Relative level of LMO2 protein expression. The P value determined by Mann-Whitney test is indicated. (C) Correlation between levels of relative protein expression of LMO2 (vertical axis) and gene expression of LMO2 analyzed by real-time RT-PCR using the primers for exons 4 and 5 (horizontal axis).