EGFP expression in HSCs of Tg(runx1P2:EGFP) and mCherry in the endothelium of Tg(kdrl:nls-mCherry) transgenic embryos. (A) Overlaid brightfield and fluorescent images of Tg(runx1P2:EGFP) at 48 hours postfertilization (hpf) displaying rounded HSCs (arrowheads in higher magnification of boxed area) just ventral to the DA. (B-C) Lateral views of Tg(kdrl:nls-mCherry)/Tg(fli1a:EGFP) compound transgenic embryos at 24 and 48 hpf. Red fluorescent mCherry was localized to the nuclei of all endothelial cells of the DA, CV, and intersegmental vessels (ISV) of the trunk, and numerous endothelial cells of the caudal vascular plexus (arrows). Scale bars represent 40 μm.