Depletion of Runx1, Vegfa, or Tnnt2 impairs development of double-fluorescent (Runx1/EGFP, Kdrl/mCherry) HSCs in the AGM region. After MO injection, double-fluorescent cells (yellow) in the DA were scored as a percentage of total cells in the DA per field of view in a total of 5 embryos. (A) Quantification and (B-C) lateral views of representative embryos. Error bars represent SD. Statistical significance was measured by P value (Student t test). For each test bar (Runx1 MO, Tnnt2 MO, Vegfa MO), asterisks denote P values for comparisons with Ctrl MO for that developmental stage. Capped lines with P values denote comparisons between Runx1 MO with either Tnnt2 MO or Vegfa MO.