DC-10 express tolerogenic markers. (A) Monocyte-derived iDCs, DC-10, and mDCs were analyzed by flow cytometry to determine levels of expression of ILT2, ILT3, ILT4, HLA-G, and ICOS-L. Numbers represent mean fluorescence intensity. One representative donor of 10 donors analyzed is shown. (B) Mean percentages of positive cells, set according to the isotype-matched controls, gated on CD11c⁺ cells (not shown), mean ± SD are shown. *P < .01, DC-10 vs iDCs and mDCs. Mean of 10 different donors. (C) DC-10 present in peripheral blood express the tolerogenic markers ILT2, ILT3, ILT4, and HLA-G. Peripheral blood was analyzed by flow cytometry to determine levels of expression of ILT2, ILT3, ILT4, and HLA-G. Analyses were performed on gated CD14⁺CD16⁻ cells (inflammatory monocytes), CD14^lowCD16⁺ cells (resident monocytes), CD14^brightCD16⁺ cells (containing DC-10), myDCs (CD1c⁺CD11c⁺), and pDCs (CD11c⁻CD303⁺). Black histograms represent staining with the appropriate control mAbs. Data from one of 8 independent donors analyzed are presented. Percentages of CD14⁺CD16⁻, CD14^lowCD16⁺, CD14^brightCD16⁺, CD1c⁺CD11c⁺, CD303⁺CD11c⁻ cells expressing the indicated markers are shown. (D) Mean percentages of positive cells, set according to the isotype-matched controls (not shown), gated on CD14⁺CD16⁻ cells (inflammatory monocytes), CD14^lowCD16⁺ cells (resident monocytes), CD14^brightCD16⁺ cells (containing peripheral DC-10), myDCs (CD1c⁺CD11c⁺), and pDCs (CD303⁺CD11c⁻), mean ± SD are shown. *P < .01, CD14^brightCD16⁺ cells (containing peripheral DC-10) vs inflammatory and resident monocytes, and to myeloid and pDCs. Data are mean of 8 different donors.