Antibody blockade of IL-23 signaling results in a reduction in GVHD without abrogation of the GVL effect. (A) Whole body distribution of A20-luc cells over the first 60 days after transplantation using in vivo BLI is shown in animals transplanted with B6 BM only, B6 BM and spleen cells treated with isotype control antibody, or B6 BM and spleen cells treated with p19 antibody. Data are derived from 2 experiments. (B) Lethally irradiated Balb/c mice were administered 5 × 105 A20-luc+ tumor cells and the next day were transplanted with either B6 BM cells alone (□, n = 13) or together with B6 spleen cells adjusted to yield 0.225 to 0.4 × 106 T cells. Mice that received adjunctive spleen cells were then administered either isotype control (■, n = 17) or p19 antibody (○, n = 19) on the schedule as described in “Reagents.” Survival is shown. Data are cumulative results derived from 3 independent experiments. (C) Pathologic scores in individual GVHD target organs and composite score of all 3 tissues from surviving mice transplanted with p19 antibody are shown. Data are presented as mean ± SEM.
