Figure 2
Peg-Arg I impairs cyclin D3 expression in T-ALL cells. (A) Cyclin D3 expression was determined by Western blot in CCRF-CEM cells cultured with or without peg-Arg I. (B) CCRF-CEM cells (1 × 105) previously transfected with shRNA-coding plasmids against nonrelevant sequences (Control) or cyclin D3 (Cyc D3-shRNA), or transfected with empty plasmids (Empty) were cultured for 72 hours and proliferation tested by MTT. (C) CCRF-CEM cells over-expressing cyclin D3 (Cyclin D3-eGFP), expressing eGFP, or wild-type cells were cultured for 72 hours in RPMI medium (Control) or medium containing peg-Arg I (2 IU/mL), after which proliferation was tested by MTT. Experiments were repeated at least 3 times obtaining similar results.
