Figure 1
![Figure 1. Low levels of PDI are expressed on the surface of ECs. Confocal analysis of PDI immunostaining was performed on nonpermeabilized (A) or Triton X-100-permeabilized (B) EA.hy926 cells and compared with an irrelevant rabbit IgG or secondary antibody alone. Scatter plot representation and statistical analysis (one-way analysis of variance [ANOVA]) of MFIs of more than 50 individual cells per experimental condition are shown on the right. (C) Flow cytometric analysis of PDI immunostaining on surface or Triton-permeabilized ECs, shown as MFI ± SEM. ***P < .001, unpaired t test.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/116/6/10.1182_blood-2009-10-249607/4/zh89991055410001.jpeg?Expires=1735522754&Signature=rZ~KyOmFe~4AwpuIkAjXVFhi-6vIg-b-Z1k~FAp0x-NSQKpdlqyukO8GZ~LV59FoweqAKvTBS0957Q56uK8kUoEtil2LkYhHJ9N25Fn3h945xYxhDWPuqiuwSJLKz7qhihTp0ONxZhwTpkCYq4Ve-CmLl0PobiAR-ECXtpf4zwzeSjJSZxe063muLqJLcfMdg05isaVLBQQALVyBHcAT66pwCNJWqpZqs0~UcoNYUmKTM~YBWVvmr2yMuXx9XMGKClv0Dc6t2EIOJ1BZiY9p5W0kIguwZqFUfUm3OSqFFPEq5Dx7iPz~Iz4ky4KNl5B8AndhOXWSQV3RAqCBUaYttg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Low levels of PDI are expressed on the surface of ECs. Confocal analysis of PDI immunostaining was performed on nonpermeabilized (A) or Triton X-100-permeabilized (B) EA.hy926 cells and compared with an irrelevant rabbit IgG or secondary antibody alone. Scatter plot representation and statistical analysis (one-way analysis of variance [ANOVA]) of MFIs of more than 50 individual cells per experimental condition are shown on the right. (C) Flow cytometric analysis of PDI immunostaining on surface or Triton-permeabilized ECs, shown as MFI ± SEM. ***P < .001, unpaired t test.
