Extracellular PDI inhibition does not induce a detectable calcium influx in ECs. Flow cytometric analysis of calcium influx in EA.hy926 cells using Fluo-3 calcium probes. The fluorescent profiles are indistinguishable between BD34 and isotype IgG-treated cells (A) but shifted by ionomycin in both control EC (B) and anti-PDI treated ECs (C). (D) MFI ± SEM representation of panels A through C.