Figure 1
![Figure 1. Treatment of anti-D with EndoS does not affect the binding of anti-D to RBCs. (A) Western blot analysis of anti-D on RBCs using peroxidase-conjugated anti–human IgG. (B) Glycan hydrolysis of anti-D on RBCs determined by lectin ELISA (optical density [OD] = 450 nm). (C) Quantification of RBC-bound anti-D by ELISA (OD = 450 nm) using antiserum against human IgG.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/115/24/10.1182_blood-2009-08-239020/4/zh89991053770001.jpeg?Expires=1735831345&Signature=zTu8ojaIPyrQT2TR2y9~o51ZmPKI2OK3VnDSckwQMsVjldgyca2TFvA5T4fY9juMxT6VeMAYfVXSgJO8ec~tIe1rO1GD8IfITyMVAh39DEdjJpG-04U-nU1D4l6cXCjzWxTH8Un2DOrMzfnY007Dd20a8FlU60~JNlXigzaTVwWjlegFBfFA0~wpGxjhChtHv6wRRDpvmASODKkv2H3pp73wjGATMi5DfZmAYa9lnCPpULf-BraGgQGwX2Po2BaU0cVBnQGszjJ3vdOB7ZjCjniKpILJ5q1h6Sn2XFBSg24-HgWeFbDa9wK0jMuUc9xOE~eP~2UwjEwm0ePdq6X2nA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Treatment of anti-D with EndoS does not affect the binding of anti-D to RBCs. (A) Western blot analysis of anti-D on RBCs using peroxidase-conjugated anti–human IgG. (B) Glycan hydrolysis of anti-D on RBCs determined by lectin ELISA (optical density [OD] = 450 nm). (C) Quantification of RBC-bound anti-D by ELISA (OD = 450 nm) using antiserum against human IgG.
