IVM analysis of lymphocyte rolling and shear-resistant firm adhesion in mAb-blocked WT and ICAM-1^−/−, ICAM2^−/− and ICAM-1/2^−/− PLN microvasculature. (A) Rolling (top panel) and sticking fractions (bottom panel) of lymphocytes in WT PLN microvessels as a function of vessel diameter and in the presence of blocking antibodies against ICAM-1 or ICAM-2. Each dot represents 1 venule. Pooled from 3 (+anti–ICAM-2), 18 (+anti–ICAM-1), and 27 (no Abs) WT animals per condition with 5 (+anti–ICAM-2), 59 (+anti–ICAM-1) and 126 (no Abs) venules analyzed. (B) Sticking fractions of lymphocytes in WT PLN microvessels as in panel A. Each dot represents 1 venule. Bars represent mean values. n.s. indicates not significant. ***P < .0001. (C) Rolling (top panel) and sticking fractions (bottom panel) of lymphocytes in WT, ICAM-1^−/−, and ICAM-1/2^−/− PLN microvessels as a function of vessel diameter. Each dot represents 1 venule. Pooled from 6 ICAM-1^−/−, 6 ICAM-1/2^−/−, and 27 WT animals with 45 (ICAM-1^−/−), 42 (ICAM-1/2^−/−), and 126 (WT) venules analyzed. (D) Sticking fractions of lymphocytes in WT, ICAM-2^−/− (4 animals, 13 venules analyzed), ICAM-1^−/−, ICAM-1^−/− + anti–ICAM-2 (4 animals, 29 venules analyzed) and ICAM-1/2^−/− PLN microvessels as in panel C. Each dot represents 1 venule. Numbers of WT, ICAM-1^−/−, and ICAM-1/2^−/− animals and analyzed venules as in panel C. Bars represent mean values. n.s. indicates not significant. *P < .05; ***P < .0001.