8-NH2-Ado inhibits Akt and Erk signaling pathways of MCL cell lines. JeKo, Mino, SP-53, and Granta 519 cells were incubated continuously with 3μM 8-NH2-Ado for 0, 5, and 17 hours. Cell lysates (30 μg) were immunoblotted for phospho- and total protein levels as shown. GAPDH was used as a loading control.