Dll4 does not inhibit early steps of MK differentiation. Human cord blood CD34⁺ cells were plated onto Dll4Fc-coated plates for 5 days. (A) FACS analyses for CD34 and CD41a markers of 1 representative experiment. (B) Histogram representation of the % of CD34⁺ cells at day 5 of the culture. Mean ± SEM from 9 independent experiments. (C) Total nucleated cells were plated on plasmatic coagulum for CFU-MK determination. Histogram representation of the number of CFU-MK in 1500 mononuclear cells (mean ± SEM from 4 independent experiments).