SLP-76 is required for normal memory generation after acute LCMV infection. (A) Schematic of the experimental design. cHET and cKO mice were infected with LCMV-Armstrong on day 0, with subsequent expansion of CD8⁺ T cells (bold line). On days 11 to 15, mice were administered tamoxifen with subsequent deletion of SLP-76 and expression of YFP (dashed line). (B) Contour plots of CD8⁺H-2D^b:GP33⁺YFP⁺ gated peripheral blood lymphocytes isolated at 1 and 2 weeks after deletion from cHET and cKO mice. Frequencies in each gate are indicated in the top right corner of each plot. Data are representative of 5 cHET and 5 cKO mice. (C) Longitudinal quantitation of CD8⁺H-2D^b:GP33⁺YFP⁺ KLRG1^lowCD127^high (left) and KLRG1^highCD127^low (right) frequencies from cHET versus cKO peripheral blood lymphocytes. Mean and standard deviations are a composite from 2 independent experiments using a total of 5 cHET and 5 cKO mice. (D) Contour plots from splenocytes isolated from cHET and cKO mice 4 weeks after deletion. All plots are gated on CD8⁺H-2D^b:GP33⁺YFP⁺. Shaded histograms are from cHET mice; solid black histograms are from cKO mice. Data are representative of 2 independent experiments. (E) Quantitation of CD8⁺H-2D^b:GP33⁺YFP⁺ MP and SLEC populations in cHET and cKO mice spleens 4 weeks after deletion. Each point represents an individual mouse.