Cre is expressed and Bax is deleted in early thymocyte progenitor populations. (A) Immunofluorescence of thymocyte cells from LckCre-BaxF/⁺Bak^−/− (control) and LckCre⁺BaxBakDKO mice. (B) Real-time PCR data confirming the expression of Cre in DN T-cell populations sorted as described in “Methods.” (C-D) Relative expression of intracellular Bax in DN1c-kit^high cells of control and LckCre⁺BaxBakDKO mice as measured by flow cytometric analysis. (E-F) Relative expression of intracellular Bax in DN3 cells of control and LckCre⁺BaxBak DKO mice by flow cytometric analysis.