Survival curve and disease phenotypes of LckCre⁺BaxBak DKO mice develop T-ALL. (A) Kaplan-Meier survival curves for cohorts of LckCre⁺BaxBak DKO and control mice. The median survival of LckCre⁺BaxBak DKO mice was 10 months. n = 21 for both cohorts of mice, and P = .0001. (B) PCR of genomic DNA of LckCre⁺BaxBak DKO tumors for rearrangements at the TCRβ locus using Dβ2 and Jβ2 region-specific primers. LckCre⁺BaxBak DKO tumors display clonal beta chain rearrangement. (C) Representative immunophenotype of T-ALL arising in LckCre⁺BaxBak DKO mice. (D) Blood smear and cytospin of BM from a representative LckCre⁺BaxBak DKO tumor.