LckCre⁺BaxBak DKO tumors do not demonstrate activation of the Notch pathway. Total RNA was isolated from tumor and healthy T cells from LckCre⁺BaxBak DKO and wild type mice. Real-time PCR analysis was performed and expression levels of the Notch1 signaling pathways genes; Notch1, Deltex1, hairy-enhancer of split 1 were normalized with actin and compared. LckCre⁺BaxBak DKO tumors that demonstrate efficient excision of Bax do not demonstrate evidence of activated Notch signaling. The horizontal bars indicate the medium value, and the black points (■ healthy WT; ▴ healthy DKO; ▾ tumor Wt;♦ tumor DKO) delineate the overall range of expression of the indicated genes. **P < .05, and ***P < .01.