Figure 1
![Figure 1. Identification of a novel D156Y PCFT mutation in a subject with HFM and its expression as well as transport function. (A) A chromatogram showing a homozygous mutation in the PCFT gene in a subject with HFM. (B) [3H]-MTX (0.5μM) influx over 1 minute at pH 5.5 and 37°C in Hela-R1-11 cells transiently transfected with wild-type PCFT, D156Y-PCFT, or the vector (mock). (C) Western blot analysis of cells transiently transfected with wild-type PCFT, the D156Y mutant, or vector: crude cell membrane fraction (left); biotinylated protein at the cell surface (right). Vertical lines have been inserted to indicate repositioned gel lanes.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/116/24/10.1182_blood-2010-06-291237/4/zh89991061410001.jpeg?Expires=1735519821&Signature=Ua5pcowMbaJVjYHfMi7x~cCS2YT0A6dQimy33qOhzEkgPpeYP2t1Qq2J0mLX8wmbjE8aLvwH~B4Vtt8KE3vvbEmD6iy4pXJLGzMPADEtoltXk~AYE3hZdDEn8Of2vs5u~hf2mSNZxYnMfQGZw0gqz34LDVMxKBIENwnlGTuQhpL7PHn0t7Bj6APiQXV1A1SCZziqi0h7z2f8tZYT8Zxt5JWEbOtodaMO26Wemvof4nGBse6YMcDXtgwNgT1XdYnrhzkQwvmqwNLjSQZtr~is4Nppe76GYghyG5KqGNrd2THUCVcDP4XFZhwLtkMoQnOvFV4ThiyY-SjIOoo~ixnG5Q__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Identification of a novel D156Y PCFT mutation in a subject with HFM and its expression as well as transport function. (A) A chromatogram showing a homozygous mutation in the PCFT gene in a subject with HFM. (B) [3H]-MTX (0.5μM) influx over 1 minute at pH 5.5 and 37°C in Hela-R1-11 cells transiently transfected with wild-type PCFT, D156Y-PCFT, or the vector (mock). (C) Western blot analysis of cells transiently transfected with wild-type PCFT, the D156Y mutant, or vector: crude cell membrane fraction (left); biotinylated protein at the cell surface (right). Vertical lines have been inserted to indicate repositioned gel lanes.
