Figure 4
![Figure 4. Expression and function of D156 PCFT mutants. (A) [3H]-MTX influx was performed at 0.5μM and pH 5.5 over 1 minute. Data are the mean ± SEM from 3 independent experiments performed on different days. (B) Western blot assay of the crude cell-membrane fraction (top panel) and biotinylated protein at the cell surface (middle panel). β-actin was the loading control (bottom panel). These blots are representative of 3 experiments and were performed on 2 gels at the same time.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/116/24/10.1182_blood-2010-06-291237/4/zh89991061410004.jpeg?Expires=1735517733&Signature=rB8rsD4RIsnM3gCEwTjherfF3NCTeTuYWGOvsFSBOeCvmfuzq4t4fPU2dlOlI8O~Zvji4l1CNtFH8DaP2QaRLGXrpa991SIfCSVfnVTKCTFu3EEkIVVODr2p3Nx~CPmUm0phLqQs5xqJg9VteMe5RO2ZlOaukAxRclfQJ45kAyycC9f-8mzEBZyPXi8Jv9TOCxLGGbom4GaTBdcKZYM~03riRFYSE-Xi52g8LyZWdAhQkqpRZu4zbw~rgQhMI5ftMwKUBZIW2zr2coTMm5vUmcpNLblV8NJDYSGmEwtiYWZ490nd1s1YIYOUKir30cN-c8ILhaJIuyt9h8ghW6eaCA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Expression and function of D156 PCFT mutants. (A) [3H]-MTX influx was performed at 0.5μM and pH 5.5 over 1 minute. Data are the mean ± SEM from 3 independent experiments performed on different days. (B) Western blot assay of the crude cell-membrane fraction (top panel) and biotinylated protein at the cell surface (middle panel). β-actin was the loading control (bottom panel). These blots are representative of 3 experiments and were performed on 2 gels at the same time.
