Figure 6
![Figure 6. Functional properties of D109 PCFT mutants. (A) [3H]-MTX influx (0.5μM) over 1 minute at pH 5.5. Data are the mean ± SEM from 3 independent experiments. (B) Western blot assay of the crude cell-membrane fraction (top panel) and biotinylated protein (middle panel) of D109 PCFT mutants. β-actin was the loading control (bottom panel). The graph shown is a representative of 2 independent experiments. The vertical line between the D109S and Mock lanes indicates repositioned gel lanes.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/116/24/10.1182_blood-2010-06-291237/4/zh89991061410006.jpeg?Expires=1735517911&Signature=KIsJag4liLlWzJT2tU9X9bXaMedmFx0jeIrkfjZeUggJf53B7f0ndiooIep35OlIqaDDTqwe3cAjI3Uw9YtaR0lLUJabHXOOqZVHZR0pAnalSOkodYifSMcHTu1qsf3lX2xC4ds20Dphexg19j~6ggzBDPXT-ly063-m9Vh5pZh34qZOS8OM4f9Yd9ljKt8FVqRyqiAckSwKYpazj-x-~LtCDAYoeMDLjhhsB8am67~jzuqX1XSrBGIX6TKLxmiwTgYfFBHmHJRo9V5KfPH7jLV9pByXt42iw1ghSljFP5NJVmPzSD3xr~GtXhn-HMcAr9btxQOCGZXugUqjmJ5q6A__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Functional properties of D109 PCFT mutants. (A) [3H]-MTX influx (0.5μM) over 1 minute at pH 5.5. Data are the mean ± SEM from 3 independent experiments. (B) Western blot assay of the crude cell-membrane fraction (top panel) and biotinylated protein (middle panel) of D109 PCFT mutants. β-actin was the loading control (bottom panel). The graph shown is a representative of 2 independent experiments. The vertical line between the D109S and Mock lanes indicates repositioned gel lanes.
