Figure 7
![Figure 7. Impact of MTX concentration and pH on the activity of PCFT D109 mutants. (A) [3H]-MTX influx (10μM) over 1 minute at pH 5.5. (B) [3H]-MTX influx (100μM) at pH 5.5. (C) [3H]-MTX influx (0.5μM) at pH 4.5. (D) [3H]-MTX influx (100μM) at pH 4.5.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/116/24/10.1182_blood-2010-06-291237/4/zh89991061410007.jpeg?Expires=1735519741&Signature=ou301TwbX2uF0uqyqDOpriw1tDubUxE9YXXJfEyE7mhONt4uGo1lVyyKLpTZTBq2Pmr7QCuNrq5DbMpBh5lcxNgtXlUFfnALFMtLTIwHVkYRS0P7uaYFQ~TI9dw3GjAYUGM3uRQksOVWgZ2pwNbX63flZxsFFTMuAgn9vSX2n2fBVZK0sQ3mdEjHT5XkbdqG-tJhwjyF7JIoimzf0RArqoV3JcrbBvzyEldh8Pkfh1buTDUxupJQzWZ~uY2wrfh7sfX9cdbsEuGI8FZtphDwXUjyHwfh0pgikv3GxOQ4A6LkNF6P6Is2v1-MD6BD04DrL85di~5jMEnm-cTHt04lyg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Impact of MTX concentration and pH on the activity of PCFT D109 mutants. (A) [3H]-MTX influx (10μM) over 1 minute at pH 5.5. (B) [3H]-MTX influx (100μM) at pH 5.5. (C) [3H]-MTX influx (0.5μM) at pH 4.5. (D) [3H]-MTX influx (100μM) at pH 4.5.
