Constitutive Ras activation promotes mTORC1, mTORC2, and PI3K/Akt signaling. Thymocytes from WT and caKRas-T (KRas) mice were either not pretreated or pretreated with rapamycin (Rapa) for 30 minutes at 37°C and were left unstimulated or stimulated with an anti-CD3 antibody (500A2) at 37°C for 2 or 10 minutes, respectively. Cell lysates were separated by SDS-PAGE followed by immunoblotting with the indicated antibodies. The blots were stripped and reprobed with an anti–β-actin antibody for a loading control. Data shown and bar graphs are representative of/quantified from 3 experiments. *P < .05; **P < .01; ***P < .001 indicate statistical significance between WT and KRas after anti-CD3 stimulation.