c-myb:EGFP⁺ cells show defective polyadenylation of snrnp70 in grechetto mutants. (A) Schematic representation of the poly(A) length assay, described in “Poly(A) tail assay.” (B) Poly(A) length assay for snrnp70 in c-myb:EGFP⁺–sorted cells from grechetto mutants (lane 2) or WT siblings (lane 1) shows defective polyadenylation of the transcript in the mutants. Control gene-specific RT-PCR showed the presence of the snrnp70 transcript in both grechetto mutants (lane 4) and WT siblings (lane 3).