CCR7^−/− HSCs demonstrate enhanced myeloid reconstitution after HSCT. (A) Experimental scheme of a competitive reconstitution assay in which WT CD45.1 mice were lethally irradiated and reconstituted with 3.0 × 10² HSCs from WT CD45.1 and 3.0 × 10² HSCs from either WT (CD45.2) or CCR7^−/− (CD45.2) mice, and 1.0 × 10⁴ CMPs and GMPs from CD45.1 WT mice. Fourteen days after HSCT, flow cytometric analysis revealed a significant increase in the percentage and total number of spleen and lung cells derived from CCR7^−/− HSCs, compared with WT HSCs. (B) Total number of cells and flow cytometric analysis of CD11b^hi and Gr-1^hi cells in the spleen 14 days after competitive transplantation. (C) Total number of cells and flow cytometric analysis of CD11b^hi and Gr-1^hi cells in the lung 14 days after competitive transplantation (n = 5 mice per group). *P < .05, when comparing cells originating from either WT or CCR7^−/− HSCs.