Lethally irradiated WT mice reconstituted with CCR7^−/− HSCs and MPCs (CCR7^−/−→WT) show increased leukocyte influx into the lungs before and after A fumigatus challenge, compared with WT→WT chimeras. Fourteen days after HSCT, mice were left unchallenged or were injected with 2.0 × 10⁶ conidia. Cellular infiltration into the lung was analyzed 48 hours after challenge. (A) Myeloid DCs were CD11c high, CD11b high, and MHC II⁺ cells. Neutrophils were CD11b high, Gr-1 high, and CD11c⁻. Monocytes/macrophages were CD11b⁺, F4/80⁺, and CD11c⁻. Plasmacytoid DCs were B220⁺ and CD11c⁺, CD4⁺ T cells were CD3⁺ and CD4⁺, and B cells were B220⁺ and CD11c⁻. (B) Dot plots of DCs and neutrophils demonstrate increased percentages of myeloid effector cells during IA (n = 4-5 mice per group. Data are representative of 3 separate experiments). *P < .05, when comparing WT or CCR7^−/−→WT chimeric mice.