CCR7^−/− HSCs and MPCs exhibit enhanced myeloid reconstitution, compared with WT cells during IA after a competitive reconstitution transplantation. (A) Experimental scheme in which WT CD45.1 mice were lethally irradiated and reconstituted with 3.0 × 10² HSCs, 5.0 × 10³ CMPs, and 5.0 × 10³ GMPs from CD45.2 WT GFP⁺ and CCR7^−/− mice. Fourteen days after HSCT, mice were challenged with 2.0 × 10⁶ conidia and cells were analyzed by flow cytometry 48 hours later. (B) Percent of total, (WT and CCR7^−/−) donor neutrophils or DCs in the lungs of chimeric mice after conidial challenge (gray bars) and the percentage of donor cells derived from WT or CCR7^−/− mice (white and black bars, respectively). (C) Contour plots showing donor-derived DCs from WT GFP⁺ or CCR7^−/− mice (n = 5 mice, representative of 2 experiments). *P < .05, when comparing GFP⁺ and CCR7^−/− donor cells after HSCT.