Fancd2^−/− mice have a smaller KSL stem and progenitor pool. (A) FACS profiles after KSL staining of the bone marrow cells from Fancd2 mutant mice and wild-type littermate controls. The percentage of the KSL gate is referring to the proportion of KSL cells in the whole nucleated bone marrow. To confirm the purity of double-sorted KSL cells, 3000 cells were analyzed for each genotype. (B) Quantification of hematopoietic KSL stem and progenitor frequencies in the bone marrow of FA mice (Fancd2^−/− or Fancc^−/−) and wild-type controls. All the mice used were between 3 and 8 months of age. In the Fancd2^−/− group, n = 19 for Fancd2^−/− mice and n = 12 for Fancd2^+/+ littermate controls; in the Fancc^−/− group, n = 6 for Fancc^−/− mice and n = 4 for Fancc^+/+ littermate controls. NS denotes not significant. (C) Quantification of CD34⁻KSL hematopoietic stem-cell frequencies in the bone marrow of Fancd2^−/− mice or wild-type controls. N = 6 for Fancd2^−/− mice and n = 7 for Fancd2^+/+ mice. (D) Quantification of CLP frequencies in the bone marrow of Fancd2^−/− mice or wild-type controls. N = 5 for Fancd2^−/− mice and n = 3 for Fancd2^+/+ mice.