Fancd2^−/− KSL cells have abnormal cell cycle status. (A) Representative cell-cycle profiles of KSL cells from a Fancd2^−/− mouse and its wild-type littermate control. DNA content was measured with Hoechst 33342. Data were analyzed with FlowJo (TreeStar) using the Dean-Jett-Fox model for the quantification of each cell-cycle phase. (B) Pooled results from cell cycle analysis on KSL cells. N = 9 for each group. (C) Quantification of the cell cycle analysis on CD34⁻KSL cells. N = 3 for each group. (D) Representative picture after costaining for DNA content (Hoechst 33342) and Ki67 expression in KSL cells from a Fancd2^−/− mouse and its wild-type littermate control. Percentages for each gate were denoted.