Overexpression of miR-34a in AML blast cells leads to granulopoiesis. Bone marrow cells derived from AML patients with CEBPA mutation (14 and 11 of Table 1) were transfected with control or miR-34a lenti viral vectors. Cells were cultured for 6 days and collected for morphologic, immunophenotypic, and myeloid marker expression analysis. (A) Morphologic analysis by light microscopy of Wright-Giemsa–stained AML blast cells. (B) miR-34a expression levels in blast cells transfected with lentiviral miR-34a vector, in comparison with control vector, as analyzed by real-time RT-PCR analysis. (C,E) CD11b (C) and CD14 (E) expression levels in blast cells transfected with lenti viral miR-34a vector, in comparison with control vector, as analyzed FACS analysis. (D,F) G-CSFR (D) and M-CSFR (F) expression levels in blast cells transfected with lenti viral miR-34a vector, in comparison with control vector, as analyzed by real-time RT-PCR analysis. Data are represented as mean ± SD from 3 independent experiments. *P < .05.