Three representative common abnormalities seen in AML compared with normal BM. (A) Constitutive activation difference: single parameter histogram overlays of pStat5-PE intensity for untreated normal BM lymphocytes (black) and CD34⁺CD117⁺ cells (red) compared with a similar overlay for untreated AML#1. (B) Dysregulation showing increased activation. pAkt-A647 overlay of unstimulated (black) and GM-CSF–stimulated (red) CD34⁺CD117⁺ cells comparing results for a representative normal BM and AML#9. (C) XY-Scatter plot of pAkt percentage responding CD34⁺CD117⁺ cells versus time poststimulation showing kinetic differences between the representative normal and abnormal AML#7 CD34⁺CD117⁺ cells. Acquired on Gallios flow cytometer and analyzed with FCS Express Version 3.