Function of CB6F1 NK cells subsets defined by Ly49. (A) NK cells from peripheral blood of 8-week-old BALB/c, B6, or CB6F1 mice were stained for their Ly49 repertoire. (B) F1 NK cells gated on Ly49A⁻ cells. (C) Enriched splenic NK cells from F1 mice were stimulated with plate-bound antibodies in the presence of anti-CD107a. Bar graph demonstrates mean and SD of triplicate wells. (D) F1 NK cells were incubated with equal numbers of β2^−/− lymphoblast blasts in the presence of anti-CD107a. *P < .005 for Ly49A or Ly49G2 versus Ly49C/I subset, no significant difference between Ly49G2 and Ly49A subsets. Data shown are representative of 2 independent experiments.