Figure 1
Identification of TfR2 as an EpoR binding partner. (A) Strategy for identification of EpoR partners. At the end of the purification process, proteins were directly digested on the beads by trypsin, peptides were recovered and analyzed by mass spectrometry using a LTQ-Orbitrap. (B) Detection of the EpoR at each step of the purification process by Western blotting. Material corresponding to 5 × 105 cells from purification steps indicated in panel A was analyzed by Western blot using anti-EpoR antibodies. EpoR 1: cell surface form of the EpoR; EpoR 2: precursor form of the EpoR. (C) Results of Mascot analysis of the mass spectrometry data. Peptides matching with TfR2 are listed.
