Quantitative RT-PCR analysis of differentially expressed genes in patients with FHL: verification of selected microarray results. Quantitative RT-PCR study of selected genes coding for Toll-like receptors (A), proteins involved in B-cell growth (B), and differentiation and proinflammatory proteins (C-D). Three (P33, P35, and P1002) of the 5 patients selected for quantitative RT-PCR analysis of the genes were also included in microarray analysis. Patients EH-05 and MW were not included in the microarray analysis. The expression level of each gene in each sample was first normalized to the expression of 18S RNA in that sample and then normalized to the expression of that gene in normal PBMCs obtained from children diagnosed as healthy.