Figure 5
![Figure 5. LPS-induced expression of IFN-β and IFN-λ1 is proportional to the number of κB sites. (A) Western blot analysis of the efficiency of siRNA-mediated RelA knockdown in HEK293-TLR4/CD14-Md2 cells using a range of siRelA concentrations (0.1hyphen]30nM). (B) Dose-dependent inhibition of IFNβ and IFN-λ1 mRNA expression in HEK293-TLR4/CD14-Md2 cells and stimulated with LPS (1 μg/mL). The x-axis shows percent RelA expression relative to control (100%) based on the densitometry analysis of Western blots. The y-axis is fold induction over mRNA expression in resting cells. Data shown are the mean + SD from a representative of 2 independent experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/116/25/10.1182_blood-2010-05-282285/4/zh89991062570005.jpeg?Expires=1735580995&Signature=PqKfKHY1ulYt21VkivVeNmNa4mEyTpIasEBxhzsPrI-qr5aaOZxnbViEb6kYrYMISwBlpsb9gRAB1pd5ejeaQvtLvPyhd-c-BeHbyTpGGOfzjBbfPGvUlleaOZSkyqg5tI7wM4K~0ehldgMww24n4gNG31IkES0i8Izk0ogJK38CiR1CNC7dP03~ZweMXPhF6i7qoHZhAMcfUQMdZKtuzMrr0t~gLMC8yyDna7NbUbewsTG5Cn0ICd2SW82Zhbj0iv4--HSyM4LHHXSpkTHJHpsP5lK4wYHqvGQnwG~zj-vrbZpko2zAFJjaC8L21pZn9Q6OJI0wrwQFUsZdyG-dnA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
LPS-induced expression of IFN-β and IFN-λ1 is proportional to the number of κB sites. (A) Western blot analysis of the efficiency of siRNA-mediated RelA knockdown in HEK293-TLR4/CD14-Md2 cells using a range of siRelA concentrations (0.1hyphen]30nM). (B) Dose-dependent inhibition of IFNβ and IFN-λ1 mRNA expression in HEK293-TLR4/CD14-Md2 cells and stimulated with LPS (1 μg/mL). The x-axis shows percent RelA expression relative to control (100%) based on the densitometry analysis of Western blots. The y-axis is fold induction over mRNA expression in resting cells. Data shown are the mean + SD from a representative of 2 independent experiments.
