Differentiation of hESC-derived CD34⁺ cells to vascular smooth muscle cells and hematopoietic cells. (A) Morphology and expression of SMC markers in hESC-derived SMCs (α-SMA and CALPONIN). Cell nuclei were stained with DAPI (blue). Scale bar is 100 μm. B. Transcriptional expression of SMC (NG2, SM22α, PDGFR-β, CALDESMON, and α-SMA) and EC (VWF and ANG2) marker genes. (C) Various hematopoietic colonies were formed from hESC-derived CD34⁺ cells in methylcellulose medium: (i) BFU-E, (ii) CFU-GM, (iii) CFU-G, and (iv) CFU-GEMM. Scale bar is 100 μm. (D) The proportion of various hematopoietic cell-type colonies in CD34⁺ cells cultured in MethoCult.